(from bovine pancreas)
Cat#ES1031 100mg
Cat#ES1032 1g
Cat#ES1033 1kg
Storage Temperature –20°C
CAS# 9001-99-4
Grade:High purity grade, for Molecular Biology
Specific activity: ≧60 units/mg of protein
Product Description
A major application for Ribonuclease A (RNase A) is the removal of RNA from preparations of plasmid DNA.In this application, the presence of DNAse activity as an impurity is a concern. The boiling-water bath method used to eliminate contaminating DNase acitivity has proven unreliable. RNAse A is an endoribonuclease that attacks at the 3’phosphate of a pyrimidine nucleotide.The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNAse A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNAse B, it is not a glycoprotein. RNAse A can be inhibited by alkylation of His12 or His119, which are present in the active site of the enzyme. Activators of RNAse A include potassium and sodium salts.
This product is stable for at least 3 years when stored properly at –20°C.
