Product Description

(from bovine pancreas)

                                  

Cat#ES1031  100mg

Cat#ES1032  1g

Cat#ES1033  1kg                                   

Storage Temperature –20°C

CAS# 9001-99-4

Grade:High purity grade, for Molecular Biology

Specific activity: ≧60 units/mg of protein

 

Product Description

A major application for Ribonuclease A (RNase A) is the removal of RNA from preparations of plasmid DNA.In this application, the presence of DNAse activity as an impurity is a concern. The boiling-water bath method used to eliminate contaminating DNase acitivity has proven unreliable. RNAse A is an endoribonuclease that attacks at the 3’phosphate of a pyrimidine nucleotide.The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNAse A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNAse B, it is not a glycoprotein. RNAse A can be inhibited by alkylation of His12 or His119, which are present in the active site of the enzyme. Activators of RNAse A include potassium and sodium salts.

This product is stable for at least 3 years when stored properly at –20°C.

Payment Terms︰ T/T or western union